NCI-H358（人非小細胞肺癌細胞）
CBP60136
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| I. General information | |
| Synonyms: | NCI-H358 |
| Background: | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung/bronchiole; derived from metastatic site: alveolus |
| Disease: | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender: | male |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 38 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,9 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H358完全培養(yǎng)基，# CBP60136M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
| Cellular Products: | lung surfactant associated protein A (SP-A) |
| Tumor Formation: | Yes, the cells produce tumors in athymic nude mice |
| Comments: | Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose. For more information, please contact Cobioer (4008-750-250). |
