HCT-15（人結(jié)腸癌腫瘤細(xì)胞）
CBP60029
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| I. General information | |
| Synonyms: | HCT-15 |
| Background: | Evidence from DNA fingerprinting indicates that this line and DLD-1 (ATCC CCL-221) are derived from the same individual; however, isoenzymology and cytogenetic data leave some doubt. |
| Species: | Homo sapiens, human |
| Tissue: | colon |
| Disease: | Dukes' type C, colorectal adenocarcinoma |
| Gender: | male |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 12 D13S317: 8,11 D16S539: 12,13 D5S818: 13 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,11 vWA: 18,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640 + 10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days. The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products: | carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days and keratin |
| Tumor Formation: | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | HCT-15 cells are CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. For more information, please contact Cobioer (4008-750-250). |
