hEpCAM/4T1
CBPG0149
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I. Background | |||
EpCAM(Epithelial Cell Adhesion Molecule,上皮細(xì)胞粘附分子,CD326)是一種跨膜 糖蛋白,在細(xì)胞的信號(hào)傳導(dǎo)、粘附、細(xì)胞增殖分化、器官形成和維持中起著重要作用。 EpCAM 基因在人胚胎期除了胸腺組織以外的幾乎所有的上皮組織中表達(dá)。在正常成人組 織中,EpCAM 在胃腸道、生殖系統(tǒng)和呼吸道的各種組織中的簡(jiǎn)單、假復(fù)層和移行上皮細(xì) 胞的基底外側(cè)面表達(dá)。EpCAM 發(fā)生功能缺失性突變會(huì)導(dǎo)致先天性簇絨性腸病,在病理?xiàng)l 件下,EpCAM 在起源于上皮細(xì)胞的癌癥中特異性表達(dá)。此外,EpCAM 在肺癌、乳腺癌、 宮頸癌和胰腺癌等多種癌癥中高表達(dá)。 | |||
II. Introduction | |||
Host Cell: | 4T1 | ||
Expressed gene: | hEpCAM | ||
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
Freeze Medium: | 90% FBS+10% DMSO | ||
Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin | ||
Mycoplasma Testing: | Negative | ||
Storage: | Liquid nitrogen | ||
III. Description of Host Cell Line | |||
Organism: | Mouse | ||
Tissue: | Mammary gland | ||
Morphology: | Epithelial | ||
Growth Properties: | Adherent | ||
IV. Representative Data | |||
Figure 1. Recombinant hEpCAM/4T1 stably expressing hEpCAM. |
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